Fig. 2

Purity of primary astrocytes certification. a The primary cultured astrocytes were labeled with fluorescent GFAP antibody. Flow cytometry was used to detect the purity of primary cultured astrocytes. There were more than 99% cells labeled with GFAP. b Primary cultured astrocytes in slides were labeled GFAP antibody binding fluorescence, DAPI fluorescent labeled nuclei. Slides were observed under the fluorescence microscope. More than 98% cells were GFAP labeled (scale bar 100 μm). Antibody: mouse anti-GFAP (1:400, Cat#MAB360, Millipore), anti-mouse Alex 488 conjugated secondary antibody (1:200, Cat#ab150117, Abcam)