Fig. 3

The iNOS and ARG1 expression and anoxic astrocyte activation. a The primary cultural astrocytes were harvested 0 h, 6 h, 12 h, 24 h, and 48 h after OGD for 6 h. Western blot was used to detect the expression of protein iNOS and ARG1. b The expression of iNOS was hardly detected in 0 h, 6 h, and 12 h after OGD, but had an over 15 times increasing after 24 h (p < 0.05). c The expression of ARG1 did not change significantly after OGD. b, c The statistical results of primary cultural astrocytes detected by Image J. Antibody: rabbit anti-iNOS (1:1000, Cat#ab17945, Abcam), rabbit anti-ARG1 (1:1000, Cat#ab124917, Abcam), rabbit anti-beta-actin (1:2000, Cat#4970, Cell Signaling Technology), anti-rabbit (1:2000, Cat#14708, Cell Signaling Technology)