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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Lipocalin-2 may produce damaging effect after cerebral ischemia by inducing astrocytes classical activation

Fig. 9

Increase of OGD induced iNOS expression in LCN2-lentivirus transfected astrocytes. Astrocytes were transfected by LVLCN2 for 6 h and treated by OGD 24 h later. Astrocytes were fixed at 24 h after 6 h OGD. The immunofluorescence showed that iNOS expression was increased in LCN2 overexpressed astrocytes after oxygen-glucose deprivation, scale bar 20 μm. Antibody: rabbit anti-iNOS (1:200, Cat#ab17945, Abcam), anti-rabbit Alex 488 conjugated secondary antibody (1:200, Cat#ab150073, Abcam), goat anti-GFAP (1:200, Cat#ab53554, Abcam), anti-goat Alex 594 conjugated secondary antibody (1:200, Cat#ab150132, Abcam)

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Journal of Neuroinflammation

ISSN: 1742-2094