Fig. 5
Neutrophil functions played vital role in the protective effects of atRA treatment. a Schematic diagram showing the time course of neutrophil depletion, atRA administration and tMCAO. b Frequency of neutrophil in peripheral blood was analyzed with flow cytometry to demonstrate the depletion efficacy of anti-Ly6G antibodies. Percentage of CD11b+CD45hiLy6G+ neutrophil among singlets were displayed. N = 3 mice per group. ***P ≤ 0.001, versus PBS-treated group in t test. c Infarct volume of mice was quantified with TTC (red)-stained coronal sections (N = 6 mice per group). ***P ≤ 0.001 in one-way ANOVA. d Neurological deficit score was assessed right after reperfusion and 1d after tMCAO (N = 6 mice per group). **P ≤ 0.01 in two-way ANOVA