Table 1 List of primary antibodies used
Antigen | Antibody (clone no.) | Dilution | Source |
|---|---|---|---|
NLRP3 | Mouse monoclonal (Cryo-2) | 1:200 | Adipogen, Switzerland |
Interleukin-18 (IL-18) | Rabbit polyclonal (H-173) | 1:1000 | Santa Cruz Biotechnology, Dallas, TX |
X-linked inhibitor of apoptosis (XIAP) | Mouse monoclonal (48/hILP/XIAP) | 1:1000 | BD Transduction Laboratories, San Jose, CA |
Caspase-1 | Mouse monoclonal (661228) | 1:1000 | R&D Systems, Minneapolis, MN |
GAPDH | Mouse monoclonal (6C5) | 1:10,000 | EMD Millipore, Billerica, MA |