Fig. 6

Overexpression of BI-1 attenuated ROS production and inflammation at 72 h post-HI. Representative picture of western blot bands (a). Quantification analysis of band intensities for ROMO1 (b), IL-6 (c), TNFα (d), and IL-1β (e). Immunofluorescent staining and quantification of the number of positively stained cells of Iba-1 with the inflammatory marker, IL-1β, in the peri-infarcted region (f). Green was for microglial staining and red was for IL-1β. Merge showed the co-localization of IL-1β on microglia. Scale bar 50 μm; n = 3/group. Data expressed as mean ± SD; an asterisk indicates p < 0.05 vs sham; number sign p < 0.05 vs vehicle; @ p < 0.05 vs Ad-TMBIM6 or scramble; n = 6/group using one-way ANOVA followed by Tukey’s multiple-comparison post hoc analysis