Fig. 7

Fluorescent staining demonstrated BI-1’s ability to reduce ROS and attenuate inflammation at 72 h post-HI. DHE staining for ROS positive cells in the cortex (a). DHE staining around ventricles for ROS positive cells (b). Immunofluorescence and MPO-positive cell counting in peri-infarcted region (c). Red was for ROS or MPO staining and blue was for DAPI. Merge showed the number of positively stained cells (a–c) (for panel a, scale bar = 50 μm; for panel b, scale bar = 100 μm; for panel c, scale bar = 25 μm; n = 3/group)