Fig. 2

Glial cell activation is reduced in the brains of PLG-depleted mice following injection with LPS. a GFAP (red) and CD11b (green) staining in PBS-CTRL ASO, LPS-CTRL ASO, and LPS-PLG ASO mouse brains. Scale bar, 1000 μm. n = 4 sections/brain, 5–7 mice/group. b GFAP (red), CD11b (green), and CD68 (white) staining and quantification in the cortex of CTRL and PLG ASO-treated mice injected with PBS or LPS (× 20 magnification). PBS-injected PLG ASO images are not shown because they are indistinguishable from those of PBS-injected CTRL ASO. CD11b, GFAP, and CD68 expression levels are increased significantly in LPS-treated animals compared to PBS-treated animals following CTRL ASO injection (***p < 0.001 for all comparisons). CD11b (**p < 0.01), GFAP (***p < 0.001), and CD68 (***p < 0.001) are decreased significantly in LPS-injected PLG-deficient animals compared to LPS-injected CTRL ASO mice. Scale bar, 100 μm. n = 4 sections/brain, 5–7 mice/group