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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Dasatinib regulates LPS-induced microglial and astrocytic neuroinflammatory responses by inhibiting AKT/STAT3 signaling

Fig. 8

Dasatinib significantly decreases LPS-induced AKT phosphorylation in primary astrocytes. a, d, g Mouse primary astrocytes were treated with dasatinib (250 nM) or vehicle (1% DMSO) for 45 min followed by LPS (1 μg/ml) or PBS for 45 min, and western blotting was conducted with anti-p-AKT/AKT, anti-p-ERK/ERK, or anti-p-STAT3/STAT3 antibodies under low-glucose conditions. b, c; e, f; h Quantification of the data in a (con, n = 10; LPS, n = 10; dasatinib+LPS, n = 10), d (con, n = 10; LPS, n = 10, dasatinib+LPS, n = 10), or g (con, n = 6; LPS, n = 6; dasatinib+LPS, n = 6). i Mouse primary astrocytes were treated with dasatinib (250 nM) or vehicle (1% DMSO) for 45 min followed by LPS (1 μg/ml) or PBS for 45 min, and immunostaining was performed with an anti-p-STAT3 antibody. j Quantification of the data in i (con, n = 622; LPS, n = 670; dasatinib+LPS, n = 463). One-way ANOVA with Tukey’s post hoc test was used to analyze significant differences. **p < 0.01, ***p < 0.001

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