Fig. 4

Diurnal changes of IκBα and RORα in cortical microglia isolated from WT and APP-KI mice. a, b The mRNA level of IκBα (a) and RORα (b) in cortical microglia of both WT and APP-KI 2-month old mice at ZT14 were examined by CAGE sequencing. Samples of each time point were pooled from 12 mice. The median (bold line) represent the mean ± SEM of three independent experiments (*P < 0.05, ***P < 0.001, Student’s t test). c Temporal changes in the mRNA levels of IκBα in cortical microglia of 2-month-old WT and APP-KI mice were quantified using real-time PCR. Samples of each time point were pooled from three to four mice. Data are represented as mean ± SEM of three independent experiments. The asterisks indicate a statistically significant difference from the WT group (***P < 0.001, two-way ANOVA test, interaction between time and phenotypes). d The average values of IκBα collapsed over the course of light phase and dark phase were calculated. Data are represented as the mean ± SEM of three independent experiments. The daggers indicate a statistically significant difference from the WT group in dark phase (†P < 0.05, two-way ANOVA test, interaction between time and phenotypes). e Temporal changes in the mRNA level of RORα in cortical microglia of both WT and APP-KI 2-month-old mice were quantified using real-time PCR. Samples of each time point were pooled from three to four mice. Data are represented as mean ± SEM of three independent experiments. The asterisks indicate a statistically significant difference from the WT group (***P < 0.001, two-way ANOVA test, interaction between time and phenotypes). f The average values of RORα collapsed over the course of light phase and dark phase were calculated. Data are represented as the mean ± SEM of three independent experiments. The daggers indicate a statistically significant difference from the WT group in dark phase (†††P < 0.001, two-way ANOVA test, interaction between time and phenotypes)