Fig. 1

Effect of Bev treatment on tumor growth and blood vessel density over time. a Experimental timetable describing when intravenous treatment (vehicle or Bev) were performed after tumor graft (D0) as well as biphotonic (2P) and widefield acquisitions. Scale bars, 400 μm. b Evolution of the tumor growth in vehicle- (n = 4) or Bev (n = 3)-treated mice. Left panel, tumor diameters measured with a fluorescence widefield microscope and normalized by the size at the first day of treatment (D14) (*p < 0.05). Right panel, tumor volumes measured by in vivo two photon microscopy at D21 and D28 (*p < 0.05). ns, not significant. c In vivo 2P high-resolution images (3D projection, 150 μm depth) showing the micro-vascularization observed inside and around tumor at D21 and D28 after grafting for untreated (upper panel) or Bev-treated (lower panel) mice. Vessels in tumor area are outlined in red, the blue arrow shows an increase in blood vessel diameter and red ones indicate cells invading the adjacent healthy brain. Scale bar 100 μm. d Blood vessel density progression (D28 normalized on D21) measured thanks to in vivo 2P imaging for tumoral (****p < 0.0001) and peritumoral (*p < 0.05) zones for vehicle (n = 5) and Bev (n = 4) treated mice