Fig. 5

Identification and quantification of CNS-resident microglia and infiltrating leukocytes in infected WT and Ifnar1^−/− mice. a Number and percentage of microglia and infiltrating leukocytes in the CNS of sham- and ZIKV-injected WT and Ifnar1^−/− mice as determined by flow cytometry (n = 3 for sham and n = 5 for ZIKV-infected). b Numbers of microglia and leukocyte subpopulations in the CNS of sham- and ZIKV-injected WT and Ifnar1^−/− mice. Microglia, inflammatory macrophages, NK cells, neutrophils, CD4+ T cells, CD8+ T cells, and B cells were identified based on the presence of surface markers. c tSNE plot of entire dataset (WT and Ifnar1^−/−, infected and sham-injected) colored by FlowSOM cluster identities, where every point is a single cell. d tSNE plots of the same dataset colored by the expression of various markers. e tSNE plots of the same dataset, split into sham-infected WT and Ifna1r^−/− mice, and ZIKV-infected WT and Ifnar1^−/− mice; colored by the expression of CD45. **p < 0.01 when compared with infected WT mice, #p < 0.05 compared with respective sham-injected mice as determined by Mann-Whitney test for panel b