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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Preactivation of Notch1 in remote ischemic preconditioning reduces cerebral ischemia-reperfusion injury through crosstalk with the NF-κB pathway

Fig. 5

In the hippocampal neuronal OGD/R model, OGD-preconditioning activated the Notch1 and NF-κB signaling pathways, and the NF-κB signaling pathway was suppressed by Notch1-RNAi. a Notch1-RNAi significantly reduced neuronal apoptosis on day 3 and day 7 following direct lethal OGD/R injury in vitro, *P < 0.05 vs. OGD/R group. b Protein bands of NICD, Hes1, p-IKK α/β, p-NF-κB p65, Bcl-2, Bax, and β-actin from Western blot analysis. c, d OGD-preconditioning significantly upregulated the expression of proteins related to the Notch1 and NF-κB signaling pathways after OGD/R. The expression of p-IKK α/β and p-NF-κB p65 was suppressed by Notch1-RNAi. Data are presented as the means ± SEM in c and d; *P < 0.05, **P < 0.01, ***P < 0.001 vs. the OGD/R group; ##P < 0.01 vs. OGD-Pre + OGD/R + sh-NC group

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