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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Chemerin-induced macrophages pyroptosis in fetal brain tissue leads to cognitive disorder in offspring of diabetic dams

Fig. 5

Macrophage recruitment by chemerin in the brain tissue. Immunofluorescence staining for F4/80 (macrophages) and MAP2 (neurons) (a) and ChemR23 and F4/80 (b) in the forebrain tissue of 18.5-day-old fetal mice and 7-day-old offspring from control, chemerin-induced diabetic dams, and chemerin-induced diabetic dams with ChemR23 knockdown mice. c Chemerin and ChemR23 protein levels in the brain tissues of 18.5-day-old fetal mice and 7-day-old offspring from controls and chemerin-induced diabetic dams (tissues from one whole brain). d Infiltrating cell rates in brain tissues of 18.5-day-old fetal mice. Macrophages, microglia, and other cell fractions were sorted by fluorescence-activated cell sorting (FACS) (five to eight fetal brains). Data are mean with 95% CI. *Microglia from chemerin-induced diabetic group vs. microglia from controls; #Microglia from chemerin-induced diabetic group with ChemR23 knockdown vs. microglia from chemerin-induced diabetic group. ^Macrophage from chemerin-induced diabetic group vs. macrophage from controls; $Macrophage from chemerin-induced diabetic group with ChemR23 knockdown vs. macrophage from chemerin-induced diabetic group. **, ##, ^^ and $$—P < 0.01. Scale bar: 50 μm

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