Fig. 6From: Chemerin-induced macrophages pyroptosis in fetal brain tissue leads to cognitive disorder in offspring of diabetic damsActivation of pyroptosis in fetal mice and offspring of diabetic dams. mRNA (a) and protein (b) levels of NOD-like receptor family pyrin domain containing 3 (NLRP3) and apoptosis-associated speck-like protein (Asc) in macrophages isolated from brain tissue of 18.5-day-old fetal mice (five to eight fetal brains). c Protein levels of pro-caspase-1, pro-interleukin (IL)-1β, and pro-IL-18 in macrophages (left) and levels of caspase-1, IL-1β, and IL-18 in the culture supernatants of macrophages (right). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as the internal control. d Active caspase-1-positive cells in macrophages isolated from the brain tissues of 18.5-day-old fetal mice. The levels of IL-1βand IL-18 (e) in the brains of 18.5-day-old fetal mice and 7-day-old offspring (pools of three whole brains). *Chemerin-induced diabetic dams vs. controls (E18.5 days), #chemerin-induced diabetic dams with ChemR23 knockdown vs. chemerin-induced diabetic dams (E18.5 days), ^chemerin-induced diabetic dams vs. controls (B7.0 days), and $chemerin-induced diabetic dams with ChemR23 knockdown vs. chemerin-induced diabetic dams (B7.0 days). * and #—P < 0.05; **, ##, ^^, and $$—P < 0.01. Scale bar: 50 μmBack to article page