Fig. 9

Effects of CCRL2 and chemR23 removal on recognition memory of offspring of diabetic dams. Detection of rearing frequency (a) and rearing times (b) of 8-week-old offspring of a normal pregnancy and of chemerin-induced diabetic dams, chemerin-induced diabetic dams with CCRL2-knockdown, and chemerin-induced diabetic dams with ChemR23-knockdown. Measurement of crossing frequency between squares (c) and frequency of crossing the center squares (d) by 8-week old offspring. e Comparative analysis of immobility time in 8-week old offspring. *Chemerin-induced diabetic dams vs. controls, # chemerin-induced diabetic dams with ChemR23 knockdown, and chemerin-induced diabetic dams with CCRL2 knockdown vs. chemerin-induced diabetic dams. ns, not significant; * and #—P < 0.05; ** and ##—P < 0.01