Fig. 1
From: Poldip2 mediates blood-brain barrier disruption in a model of sepsis-associated encephalopathy
Heterozygous deletion of Poldip2 protects against LPS-induced BBB permeability. a Schematic of the experimental design. BBB disruption following 18 h LPS (18 mg/kg, IP) or PBS treatment was assessed by Evans blue dye extravasation in Poldip2+/+ and Poldip2+/− mice. Evans blue dye was administered by intravenous injection and allowed to circulate for 30 min before animals were sacrificed. b The graph depicts Evans blue dye concentration extracted from whole brains normalized to dry brain weight. Bars represent mean ± SEM. Two-way ANOVA ***p < 0.001 vs. Poldip2+/+ + PBS, #p < 0.05 vs. Poldip2+/+ + LPS, n = 4–7 mice/group. c Representative images of whole brains following Evans blue dye extravasation