Fig. 6

SiRNA against Poldip2 blocks LPS-induced Cox-2 induction. a Confluent monolayers of RBMVECs were treated with LPS for 0, 30, 60, 120, and 180 min before cells were lysed and immunoblotting for Cox-2 was performed. β-tubulin was used as a loading control. Representative blots are shown. The graph depicts Cox-2 expression normalized to β-tubulin. Bars represent mean ± SEM of three independent experiments. Two-way ANOVA, ***p < 0.001 vs. 0 min, **p < 0.01 vs. 0 min, *p < 0.05 vs. 0 min. b Cells transfected with siRNA against Poldip2 or control were treated with LPS or PBS for 1 h before cells were lysed and immunoblotting for Cox-2 was performed. β-tubulin was used as a loading control. Representative blots are shown. The graph depicts Cox-2 expression normalized to β-tubulin. Bars represent mean ± SEM of three independent experiments. Two-way ANOVA, **p < 0.01 vs. siCtl, ^##p < 0.01 vs. siCtl + LPS