Fig. 7

Poldip2 silencing and selective inhibition of Cox2 block LPS-induced endothelial permeability. RBMVECs were grown on transwell inserts and FITC-dextran diffusion into the lower chamber was quantified spectrophotometrically to assess changes in permeability. a RBMVECs were transfected with siPoldip2 or siCtl, seeded on transwell inserts, and treated with LPS (1 μg/mL) or PBS for 3 h before incubation with FITC-dextran. The graph depicts FITC-dextran concentration expressed as fold change relative to siCtl + PBS. Bars represent mean ± SEM of six independent experiments. Two-way ANOVA, ****p < 0.0001 vs. siCtl + PBS, ^###p < 0.001 vs. siCtl + LPS. b RBMVECs were plated on transwell inserts and treated with LPS (1 μg/ml), meloxicam (10 μΜ), or both LPS and meloxicam simultaneously for 3 h before incubation with FITC-dextran. The graph depicts FITC-dextran concentration expressed as fold change relative to Ctl + PBS. Bars represent mean ± SEM of four independent experiments. Two-way ANOVA, ****p < 0.0001 vs. Ctl + PBS, ^###p < 0.001 vs. Ctl + LPS