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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: A small peptide antagonist of the Fas receptor inhibits neuroinflammation and prevents axon degeneration and retinal ganglion cell death in an inducible mouse model of glaucoma

Fig. 6

Inhibition of Fas signaling prevents the induction of multiple inflammatory pathways implicated in the pathogenesis of glaucoma. At 28 days post-microbead or saline injection, quantitative PCR was performed on neural retina isolated from saline- and microbead-injected WT mice treated (on day 0) with ONL1204 or vehicle to assess the expression of several proinflammatory genes associated with human and/or experimental models of glaucoma: a Caspase 8 and GFAP, b proinflammatory cytokines (TNFα, IL-1β, IL-18, and IL-6), c proinflammatory chemokines (MIP-1α, MIP-1β, MIP-2, MCPI, and IP10), d complement components C3 and C1Q, and e NLRP3 and TLR4. The threshold cycle values of each gene of interest were normalized to the geometric mean of two housekeeping genes (B2-microglobulin and peptidylpropyl isomerase A) and compared with the saline + vehicle control group using the comparative C method (ΔΔC). Data are presented as fold change of control ± SEM. N = 6 per group, *P < 0.05, **P < 0.01, ***P < 0,001, ****P < 0.0001

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