Fig. 1

TLR4 is upregulated in Th17 cells from MS patients and EAE mice. a Quantitative PCR analyses of TLR4 expression in CD4⁺ peripheral blood lymphocytes between CD45RA⁺ naïve cells and CD45RO⁺ memory cells from healthy volunteers (ctrl) and MS patients (n = 8 per group). a’ Quantitative PCR analyses of IL-17 and TLR4 expression in Th17 cells from peripheral blood lymphocytes between healthy volunteers (ctrl) and MS patients (n = 8 per group). b Quantitative PCR analyses of TLR4 expression in Th17 cells from peripheral blood (PB), splenocytes (SP), and lymph nodes (LN) between normal mice (ctrl) and EAE mice (n = 10 per group) 20 days after immunization. c The relative expression of TLR4 in Th17 cells from peripheral blood lymphocytes of EAE mice at different scores (n = 6 per group). d Western blot and quantification analyses of TLR4 expression during Th17 differentiation in vitro. Data are presented as mean ± standard deviation. *P < 0.05. **P < 0.01. ***P < 0.001; Student’s t test (a, b), one-way ANOVA (c, d). Data are representative of three experiments done in triplicate