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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: TLR4-RelA-miR-30a signal pathway regulates Th17 differentiation during experimental autoimmune encephalomyelitis development

Fig. 2

TLR4 deficiency suppresses Th17 differentiation in vitro. CD4+ naïve T cells from TLR4−/− or wild type (WT) mice are cultured in Th17-polarizing conditions with or without LPS for 3 days. a Western blot test of TLR4 in CD4+ naïve T cells from TLR4−/− and WT mice. b, c Flow cytometric analyses of stimulated IL-17+ cells. d ELISA of IL-17A, IL-17F, and GM-CSF in culture supernatants. e Quantitative PCR analyses of Rorγt expression in cultured cells. Data are presented as mean ± standard deviation. *P < 0.05. **P < 0.01; one-way ANOVA. Data are representative of three experiments done in triplicate

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