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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: TLR4-RelA-miR-30a signal pathway regulates Th17 differentiation during experimental autoimmune encephalomyelitis development

Fig. 3

TLR4−/− CD4+ naïve T cells are defective in promoting EAE. Rag1−/− mice transplanted with wild type (WT) or TLR4−/− CD4+ naïve T cells are immunized with MOG for EAE induction. a Mean EAE scores of mice (n = 5 per group). bb” H&E staining (b), LFB staining (b’), and MOG immunofluorescence staining (b”) of spinal cord sections from transplanted Rag1−/− mice. Arrows show lymphocyte infiltration. Scale bar, 300 μm. Quantification analyses are on the right. c Western blot and quantification analyses of MOG expression in spinal cord. d Quantification analyses of IL-17+ cells among CD4+ gated cells from peripheral blood (PB) and spinal cord-infiltrated (SCI) lymphocytes by flow cytometry. e ELISA of IL-17A, IL-17F, and GM-CSF in peripheral blood serum. f Quantitative PCR analyses of Rorγt expression in spinal cord-infiltrated lymphocytes. g, h Splenocytes and lymph node cells from mice presented (a) on day 20 are stimulated in vitro. Flow cytometric analyses of IL-17+ and GM-CSF+ cells among CD4+ gated cells (g). The concentrations of IL-17A, IL-17F, and GM-CSF in supernatant are measured by ELISA (h). Data are presented as mean ± standard deviation. *P < 0.05. **P < 0.01. ***P < 0.001; two-way repeated measures ANOVA (a), Student’s t test (bh). Data are representative of three experiments done in triplicate

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