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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: The broad spectrum mixed-lineage kinase 3 inhibitor URMC-099 prevents acute microgliosis and cognitive decline in a mouse model of perioperative neurocognitive disorders

Fig. 2

URMC-099 prophylaxis prevents microglial morphological changes following orthopedic surgery. Three-month-old mice received three doses i.p. of URMC-099 (10 mg/kg) prior to undergoing sham or orthopedic surgery. a Representative cropped and drift-corrected XYZ stacks obtained by 2PLSM (top panels), Imaris 3D surface reconstructions (middle panels), and process movement tracks (bottom panels) (scale bar = 10 μm). b Orthopedic surgery increased microglial sphericity in vehicle-treated, but not URMC-099-treated, mice. No differences were observed for mean track speed (c) nor mean track length (d). e Representative light-sheet micrographs obtained from optically cleared hippocampal sections. f Mean microglial sphericity was increased by orthopedic surgery, and URMC099 treatment prevented this. N = 4 (bd), N = 2–3 (f); results presented as mean ± SEM; *P < 0.05, **P < 0.01, versus surgery + vehicle group; repeated-measures ANOVA (bd) or one-way ANOVA (f) with Dunnett’s multiple comparison test

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