|
Primary antibodies
|
Anti-CD3
|
Dako, Hamburg, Germany
|
A0452
|
Mouse
|
1:400
|
|
Anti-Iba1
|
Wako, Richmond, VA, USA
|
019-19741
|
Rabbit
|
1:500
|
|
Anti-MBP
|
Millipore, Burlington, MA, USA
|
MAB386
|
Rat
|
1:500
|
|
Anti-Brn3a
|
Santa Cruz Biotechnology, Dallas, TX, USA
|
sc-31984
|
Goat
|
1:200
|
|
Anti-βIII-tubulin
|
Biolegend, San Diego, CA, USA
|
801201
|
Mouse
|
1:1000
|
|
Seconday antibodies
|
Alexa Fluor-555 Anti-Goat
|
Life Technologies, Carlsbad, CA, USA
|
A-21432
|
Donkey
|
1:200
|
|
Alexa Fluor-488 Anti-Rabbit
|
Life Technologies, Carlsbad, CA, USA
|
A-21206
|
Donkey
|
1:200
|
|
Cy3 anti-Rabbit
|
Millipore, Burlington, MA, USA
|
AP187c
|
Goat
|
1:500
|
|
Cy3 anti-Mouse
|
Millipore, Burlington, MA, USA
|
AP124c
|
Goat
|
1:500
|
|
Cy3 anti-Rat
|
Millipore, Burlington, MA, USA
|
AP183C
|
Goat
|
1:500
|
