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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Activated spinal astrocytes contribute to the later phase of carrageenan-induced prostatitis pain

Fig. 1

Experimental time flow. Pain threshold measurement, using a series of von Frey hairs with logarithmically increasing stiffness (0.02–2.56 g, Stoelting), was performed day − 2 and day − 1 prior to, as well as on day 14, day 21, day 28, and day 35 upon surgery (intraprostatic injection of 1% carrageenan (20 μl), day 0). The effects of intrathecal injection of different reagents on pain threshold were measured on day 14 or 35 after surgery. Some of sham and CP/CPPS mice were sacrificed for spinal cord slices for immunostaining on day 14. Others were sacrificed for prostate sampling for histology examination (H&E), spinal cord slices for immunostaining, spinal cord dorsal horn structures isolation for CXCL1 measurement by ELISA, and for pERK and ERK expression measurement by Western blot on day 35

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