Fig. 2

PMCA2 levels in the lumbar DH of female C57Bl/6NTac mice with EAE manifesting pain. Evaluation of thermal (heat) and mechanical pain sensitivity in female mice at early phase of EAE by the a Hargreaves’ test and b von Frey filament test. c PMCA2 transcript levels in the lumbar DH. d–f PMCA2, PMAC3, and PMCA4 protein levels in the lumbar DH. The graphs (upper panel) show the quantification of the band intensity in the Western blots (lower panel; two representative lanes per group). Total protein was used to normalize for experimental variations. g Stereological quantification of PMCA2/NeuN double-labeled neurons in the lumbar DH. h Total NeuN immunoreactive cell number in the lumbar DH. The number of mice in each group is shown above bars. Values represent mean ± SEM. Significantly different by independent Student’s t test with *p < 0.05 and ****p < 0.0001