Fig. 4

PMCA2 levels in the lumbar DH of C57Bl/6Ncrl mice with EAE, not manifesting pain. Assessment of the a Hargreaves test and b von Frey filament test in mice with EAE at early phase of the disease. c PMCA2 transcript levels in the lumbar DH. d PMCA2 protein levels in the lumbar DH. The right panel shows the Western blot (two representative lanes per group), and the graphs in the left panels show the quantification of the band intensity in the Western blot. The number of mice in each group is shown above bars. Values represent mean ± SEM. There were no significant differences by independent Student’s t test