Fig. 5

Inflammatory reaction in the DH of C57Bl/6NTac mice with EAE. Assessment of a the percentage of CD45⁺ cells in the DH of C57Bl/6NTac mice, analyzed by flow cytometry. b and c Representative images of H&E staining in the lumbar DH of a control and EAE mouse, respectively. d Iba1 protein levels in the lumbar DH of mice with EAE. The graphs (left panel) show the quantification of the band intensity in the Western blots (right panel; two representative lanes per group). Total protein was used to normalize for experimental variations. e and f Representative images of the DH in a transverse lumbar SC section of a control and EAE mouse, respectively, showing Iba1 immunopositive cells (green) counterstained with DAPI (blue). g GFAP protein levels in the lumbar DH of mice with EAE. The graphs (left panel) show the quantification of the band intensity in the Western blots (right panel; two representative lanes per group). Total protein was used to normalize for experimental variations. h and i Representative images of GFAP (green) and DAPI (blue) double-labeled cells in the DH of a transverse lumbar SC section of a control and EAE mouse, respectively. Arrows point at examples of Iba1 or GFAP-labeled cells. Scale bar represents 10 μm. j–l IL-1β, IL-6, and TNFα transcript levels in the lumbar DH . The number of mice in each group is shown above bars. Values represent mean ± SEM. Significantly different by independent Student’s t test with *p < 0.05, **p < 0.01, and ****p < 0.0001