Fig. 6

Inflammatory reaction in the DH of C57Bl/6Ncrl mice with EAE. a The percentage of CD45⁺ cells in the lumbar DH of C57Bl/6Ncrl mice quantified by flow cytometry. b and c Representative images showing H&E staining in the lumbar DH of a C57Bl/6Ncrl control and EAE mouse, respectively. d Iba1 protein levels in the lumbar DH. The graph (left panel) shows the quantification of the band intensity in the Western blots (right panel; two representative lanes per group). Total protein was used to normalize for experimental variations. e and f Representative images of the DH in a transverse section of the lumbar SC in a control and EAE mouse, respectively, showing Iba1 immunoreactive cells (green) double-labeled with DAPI (blue). g GFAP protein levels in the lumbar DH of mice with EAE. The graph (left panel) shows the quantification of the band intensity in the Western blots (right panel; two representative lanes per group). Total protein was used to normalize for experimental variations. h and i Representative images of a transverse lumbar SC section showing GFAP (green) and DAPI (blue) double-labeled cells in the DH of a control and EAE mouse, respectively. Arrows point at examples of Iba1 or GFAP-labeled cells. Scale bar represents 10 μm. j–i IL-1β, IL-6, and TNFα transcript levels in the lumbar DH. The number of mice in each group is shown above bars. Values represent mean ± SEM. Significantly different by independent Student’s t test with *p < 0.05 and **p < 0.01