Fig. 7

Effects of IL-1β on PMCA2 levels in pure SC neuronal cultures. a–e Agarose gels showing IL-1β, IL-6, and TNFα receptor transcripts by qRT-PCR in two distinct biological repeats of SC neuronal cultures (N1 and N2). Spleen was used as a positive control (C⁺). Negative control (C⁻) was RNA that was subject to PCR without RT to ensure the lack of contaminating genomic DNA. f–h Quantification of PMCA2 protein levels in SC neuronal cultures treated with IL-1β, IL-6, or TNFα. Graphs (upper panels) show the quantification of the band intensity in the Western blots (lower panels; two representative lanes per group). Total protein was used to normalize for experimental variations. i PMCA2 transcript levels in pure SC neuronal cultures following treatment with IL-1β. Values represent mean ± SEM. Significantly different by independent Student’s t test, *p < 0.05 and **p < 0.01