Fig. 3

MRI-DTI images and metrics of the demyelination lesion. a Representative MRI images of the mouse spinal cord acquired at the lesion level at 3, 10, and 21 dpi. Arrow indicates the dorsal injection sites for CTR, LPC, and LPS groups. Scale bar = 1000 μm. b Illustration of DTI metrics. c Illustration of alternations in DTI metrics under pathological conditions. d–g DTI metrics FA, MD, λ_ll, λ_⊥, respectively of the dorsal spinal cord at the lesion level at 3, 10, and 21 dpi. For each time-point, the values were compared to the CTR group (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) and the LPC group (××p < 0.01, ×××p < 0.001, ××××p < 0.0001); for each group, the values were compared to 3 dpi (#p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001) and 10 dpi (+p < 0.05, ++p < 0.01, +++p < 0.001). Data were collected from three animals per group at each time-point (n = 3 mice)