Fig. 3

CD73 expression on B_reg cells in the spleen and CD73 expression in the CNS. In the spleen, the number of B10 (CD19⁺CD5⁺CD1d^hi) Breg cells expressing CD73 is significantly increased in both the sham- and E2-treated IL-10 KO groups (n = 4), compared to the WT (n = 4) groups (p < 0.05; a). Another subset of Breg cells and plasmablasts (CD19⁺CD138⁺CD44^hi) had significantly more cells that were expressing CD73 in the spleens of IL-10 KO (n = 4) mice in both the sham and E2 treatment groups compared to WT (n = 4) mice (p < 0.01; b). Within the spinal cords, there was not a significant difference in CD73 expression between WT mice regardless of treatment (n = 3; c). CD73 expression was significantly increased in the spinal cords of E2-treated IL-10 KO mice compared to sham-treated IL-10 KO mice (p < 0.05; n = 3; d). Data are represented as mean ± SEM