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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Estrogen-induced compensatory mechanisms protect IL-10-deficient mice from developing EAE

Fig. 6

E2 treatment decreases nucleated cells in spinal cord white matter and decreases demyelination. Spinal cord sections were stained with Luxol Fast Blue/periodic acid-Schiff/hematoxylin and analyzed for the percentage of nucleated cells in the white matter and for demyelination. A representative section from WT sham (n = 4) mouse shows purple areas of nucleated cells and loss of myelin, indicated by blue staining (a). In the WT E2-treated (n = 4) mouse, there was an intact myelin and no dense areas of purple, nucleated cells in the white matter, demonstrated by the intact blue staining (b). The IL-10 KO sham (n = 4) mouse had several concentrated areas of purple, nucleated cells in the white matter and a loss of most of the blue staining indicating diffuse demyelination (c). The IL-10 KO E2 (n = 4) mouse had a few small areas of purple nucleated cells in the white matter and minimal myelin loss (d). Scale bar equals 100 μm. A quantitation of the percentage of nucleated cells within the white matter in each group showed E2 treatment significantly decreased the percentage of nucleated cells in the white matter compared to the sham treatment groups for both the WT and IL-10 KO mice (p < 0.01). There was also a significant increase in the percentage of nucleated cells in the white matter of the IL-10 KO sham compared to the WT sham (p < 0.05; e). E2 treatment significantly decreased the percentage of demyelination in WT and IL-10 KO mice (p < 0.05). There was also a significant increase in the percentage of demyelination in the IL-10 KO E2 mice compared to the WT E2 mice (p < 0.01; f). Data are represented as mean ± SEM

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