Fig. 7

IL-10 KO mice treated with E2 downregulate inflammatory cytokines, chemokines, and chemokine receptors in the spinal cord. The two E2-treated groups, WT (n = 3) and IL-10 KO (n = 3), tend to downregulate most of the inflammatory cytokines, chemokines, and their receptors, where the sham groups, WT (n = 3) and IL-10 KO (n = 3), tend to upregulate these same genes as shown in the cluster gram of significantly regulated genes (p < 0.05 and fold regulation of 0–2). High-throughput analysis clustered genes on the consensus of fold regulation changes into four families of genes, F1–F4 (a). When looking at only the WT sham group compared to the IL-10 KO sham group, eight genes were significantly upregulated in the IL-10 KO sham group (b). Comparing the WT E2 group to the IL-10 KO E2 group, the IL-10 KO E2 significantly downregulated 15 key inflammatory cytokines, chemokines, or receptors and significantly upregulated one gene compared to the WT E2 group (c). Data are represented as mean ± SEM