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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: NDP-MSH binding melanocortin-1 receptor ameliorates neuroinflammation and BBB disruption through CREB/Nr4a1/NF-κB pathway after intracerebral hemorrhage in mice

Fig. 5

The effects of NDP-MSH treatment and knockdown of Mc1r on expression of downstream molecules at 24 h after ICH. a Representative Western blot bands of the downstream signaling pathway protein. b-j Densitometric quantification suggested that the administration of NDP-MSH, a agonist of Mc1r, prominently upregulated the levels of p-CREB, Nr4a1, ZO-1, occludin, and laminin-α5 (Lama5) at 24 h post-ICH . In addition, treatment with NDP-MSH significantly decreased p-NF-κB p65, IL-1β, TNF-α, and MMP-9 at the same time. In contrast, the knockdown of Mc1r led to a decrease of p-CREB, Nr4a1, ZO-1, occludin, and Lama5 and an increase of p-NF-κB p65, IL-1β, TNF-α, and MMP-9. #P < 0.05 vs sham; *P < 0.05 vs ICH + vehicle; @P < 0.05 vs ICH + NDP-MSH, and ICH + NDP-MSH + Scr siRNA. Scr siRNA, scrambled siRNA

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