Fig. 1
Virus titer and cytokine/chemokine levels in the serum and organs of tick-borne encephalitis virus (TBEV)-infected mice. Female BALB/c mice were subcutaneously inoculated with ~ 500 pfu of TBEV and then euthanized on days 1, 3, 5, 7, and 9 p.i. Serum, spleen, and brain samples were collected and used for virus titration and cytokine/chemokine measurement. a Virus titer in serum samples from infected mice (dashed line; detection threshold, 1.2 log10 pfu/mL). b Virus titer in spleen and brain tissue suspensions (dashed line; detection threshold for brain, 1.8 log10 pfu/g and for spleen, 1.2 log10 pfu/g). c The levels of multiple cytokines, chemokines, colony-stimulating factors, and growth factors were determined using the MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel assay. Asterisks indicate statistically significant differences compared to uninfected controls