Fig. 4

ET-1 overexpression influences astrogenesis post tMCAO. ET-1 overexpression stimulates astrogenesis in the the SVZ of the ischemic brain at 28 days and activates phosphorylation Stat3 expression at 7 days in the ischemia brain following tMCAO. a Images show immunostaining for BrdU+GFAP+ cells in SVZ of Ntg and GET-1 mice 28 days post tMCAO. b bar graph depicting the high number of BrdU/GFAP co-labeled cells in ipsilateral SVZ of GET-1 than Ntg mice; c Images show double immunostaining for BrdU and NeuN iin SVZ cells of Ntg and GET-1 mice 28 after tMCAO. d Images show double immunostaining for BrdU and NeuN iin penumbra region cells of eNtg and GET-1 mice 28 days post tMCAO. e Bar graphs depicting the number of BrdU/NeuN co-labeled cells in ipsilateral SVZ and penumbra region of GET-1 mice than Ntg. Scale bar = 100 μm