Fig. 2

The P2Y12R antagonists MRS2395 and clopidogrel prevented NTG-induced mechanical allodynia and thermal hyperalgesia. Repeated treatment with MRS2395 blocked basal periorbital mechanical allodynia (a) and hindpaw mechanical (c) and thermal hyperalgesia (e). Post-treatment responses including periorbital mechanical allodynia (b) and hindpaw mechanical (d) and thermal hyperalgesia (f) were assessed 2 h after NTG administration. Data are presented as mean ± SEM; n = 10 per group; two-way ANOVA and Bonferroni post hoc analysis; **p < 0.01 and ***p < 0.001 compared with the sham group; ^#p < 0.05 and ^##p < 0.01 compared with the NTG group. Similarly, i.p. treatment with clopidogrel (15, 45, and 100 mg/kg; n = 10 per group) also showed an analgesic effect in mice with basal and post-treatment mechanical allodynia (g–j) and thermal hyperalgesia (k, l) after NTG injection. Values are presented as mean ± SEM; two-way ANOVA and Bonferroni post hoc analysis; *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the sham group; ^{#,$,^}p < 0.05 and ^{##,$$,^^}p < 0.01; 15, 45 and 100 mg/kg-treated groups, respectively, compared with the NTG group