Skip to main content
Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: P2Y12 receptor mediates microglial activation via RhoA/ROCK pathway in the trigeminal nucleus caudalis in a mouse model of chronic migraine

Fig. 3

Inhibition of P2Y12R function reduced NTG-induced CGRP and c-fos expression in the TNC. a Representative western blots showing the changes in CGRP expression over time in the TNC after NTG administration. The band intensities are presented relative to those of β-actin. Quantification of CGRP protein levels showed a significant increase on days 5 and 9 after NTG injection. Data are presented as mean ± SEM; n = 6 per group; *p < 0.05 compared with the sham group. b Double immunofluorescence staining images of c-fos with NeuN in sham and NTG injected mice. Scale bar: 20 μm. c, f Western blots showing that the P2Y12R inhibitors MRS2395 and clopidogrel partially suppressed the increase in CGRP (c) and c-fos (f) protein levels in the TNC; clopidogrel (15 mg/kg) showed the most significant inhibitory effect on CGRP (c). The band intensities are presented relative to those of β-actin. Values are presented as mean ± SEM; n = 6 per group; *p < 0.05 and **p < 0.01 compared with the sham group; #p < 0.05 and ##p < 0.01 compared with the NTG group. d, g Immunofluorescence staining images of CGRP (d) and c-fos (g) in the TNC. Scale bars: 100 μm for CGRP, 20 μm for c-fos. e, h Quantitative analysis of CGRP immunoreactivity (e) and c-fos-ir cells (h) in the TNC after NTG injection. The results show changes consistent with the Western blot analysis results. Data are presented as mean ± SEM; n = 4 per group; six sections from each mouse; **p < 0.01 and ***p < 0.001 compared with the sham group; ##p < 0.01 and ###p < 0.001 compared with the NTG group

Back to article page