Fig. 6

Inhibition of ROCK2 activity attenuated NTG induced CM-like behaviors. Repeated treatment with the ROCK2 antagonist fasudil significantly blocked NTG-induced basal periorbital mechanical allodynia (a) and hindpaw mechanical (c) and thermal hyperalgesia (e). Post-treatment responses, including periorbital mechanical allodynia (b) and hindpaw mechanical (d) and thermal hyperalgesia (f), were assessed 2 h after NTG administration. Data are presented as mean ± SEM; n = 10 per group; two-way ANOVA and Bonferroni post hoc analysis; **p < 0.01 and ***p < 0.001 compared with the sham group; ^#p < 0.05 and ^##p < 0.01 compared with the NTG group