Fig. 1
From: Peripheral loss of EphA4 ameliorates TBI-induced neuroinflammation and tissue damage
Neuroprotection in EphA4−/− mice and human meta-analysis data of EPHA4. a, b Nissl staining of sagittal sections from WT or EphA4 global knockout mice at 14 days post-CCI injury compared to wild type. c–h Retrospective analysis of data archived as NIH GEO datasets from human patients following brain aneurysms. This study evaluated the gene expression on > 41,000 transcripts. Superficial, n = 10; unruptured, n = 5; ruptured, n = 8. i Western blot analysis for EphA4 protein expression in CCI-injured cortical tissue at 2 h compared to sham-injured cortices (n = 3–5/group). j Representative confocal images of anti-EphA4 immuno-labeling (red; inset) at 24 h post-CCI injury in the ipsilateral cortex of CX3CR1GFP/+ mice. CX3CR1-expressing microglia and/or infiltrating monocytes/macrophages show high expression of EphA4 in the peri-lesion cortex (J1) compared to adjacent cells in the medial parietal cortex (J2). Scale bar in j = 200 μm; scale bar in (J1) and (J2) = 20 μm. ANOVA with Bonferroni post hoc test. **p < 0.01, ***p < 0.001 compared to superficial aneurysms