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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Peripheral loss of EphA4 ameliorates TBI-induced neuroinflammation and tissue damage

Fig. 3

Bone marrow chimeric EphA4−/− mice show decreased lesion volume and altered immune profile following CCI injury. a Bone marrow chimeric WTWTBMC and WTKOBMC mice were generated by irradiation and reconstitution with either wild-type or EphA4 knockout BMCs. PCR showing loss of transcript for Epha4 in the whole blood of WTKOBMC mice compared to WTWTBMC. bd WTKOBMC mice display decreased lesion volumes compared to WTWTBMC at 3 days following CCI injury. ei Max projection of z-stack confocal images of the injured cortex showing GFP+ BMCS and DAPI (blue) from WT and jn KO immune cell infiltration. o Non-biased stereological quantification showed reduced total GFP+ numbers and p co-labeled CD68/GFP-positive cells in the ipsilateral cortex of WTKOBMC mice compared to WTWTBMC. q No difference was seen in the number of Ly6G/GFP+ neutrophils. r CD45-positive enriched cell isolation from the ipsilateral cortex at 3 days showed high purity of cx3cr1 mRNA expression compared with GFAP, Neun, Tie2, and Vecad. s KO BMCs isolated from the injured cortex showed reduced pro-inflammatory CD68, MCP1, and Cxcl16 expression concomitant with t increased pro-resolving TGFβ, Tie2, Angpt1, and Angpt2. *p < 0.05, ***p < 0.001, ****p < 0.0001 compared to the corresponding WT BMCs. e and j scale bar = 500 μm; fi and kn scale bar = 50 μm

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