Fig. 5

Phosphoarray analysis of LPS-stimulated wild-type and EphA4^−/− monocyte/macrophages. a–d Phospho(p) expression from 4 h LPS- vs vehicle-stimulated WT and KO MΦ monocytes using multiplex phosphoarray. Data is represented as fold change from average six replicates in vehicle KO cells (black bars) and LPS-treated KO cells (black checkered) compared to corresponding WT treated cells (dotted line). Any fold change above 1.5 and below 0.5 was considered significant with a 95% CI and was used to quantify the precision of the phosphorylation ratio based on the analysis of the replicates. e–l mRNA analysis in MΦ, M1, and M2 cells following polarization with PBS, IFNγ, and IL4, respectively. e Loss of Epha4 transcript was confirmed in the KO cells. All M1/M2 data was normalized to WT MΦ levels. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to the corresponding control treatment