Fig. 1
From: Cellular source of hypothalamic macrophage accumulation in diet-induced obesity
Increased vascular permeability in the ARC of HFD-induced obese mice. a, b Images and quantification analysis of Evans blue leakage in the hypothalamus of mice fed on a CD or HFD for the indicated periods. c Double fluorescence images of the vascular marker PECAM1 and fluorescence (Fl)-labeled albumin in the ARC of CD- and HFD-fed mice that received an intra-tail vein injection of Fl-albumin. d Representative images and quantification analysis of fenestrated vessel marker MECA32 expression in the hypothalamic ARC during the course of HFD feeding. e Double immunostaining of MECA32 and GFP showing activated LysMGFP perivascular macrophages around MECA32+ permeable ARC blood vessels in 20-week HFD-fed mice. Scale bars, 50 μm. 3V, third ventricle; ARC, hypothalamic arcuate nucleus; MBH, mediobasal hypothalamus; ME, median eminence. Results are presented as a mean ± SEM. *p < 0.05 and **p < 0.01 vs. CD or between indicated groups