Fig. 4
From: Cellular source of hypothalamic macrophage accumulation in diet-induced obesity
Infiltration of the hypothalamus by LysMGFP cells during development. a Representative images of GFP and Iba1 double immunostaining in the brains of LysMGFP embryos and neonates at embryonic days (E) 16.5 and 18.5 and postnatal days (P) 1, 7, 14, and 28. b, c Quantification of LysMGFP cells and Iba1 alone-expressing microglia in the hypothalamus of LysMGFP embryos and neonates. Scale bars, 100 μm. 3V, third ventricle. Results are presented as a mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 between the indicated groups