Table 1 Sequences of PCR primers used in this study
From: CD147 as a key mediator of the spleen inflammatory response in mice after focal cerebral ischemia
Gene | Forward | Reverse |
|---|---|---|
CD147 | 5′-GACACTGGGGAAGAAGAGGC-3′ | 5′-GCAGTGAGATGGTTTCCCGA-3′ |
TNF-α | 5′-AGTCCGGGCAGGTCTACTTT-3′ | 5′-ACCCTGAGCCATAATCCCCT-3′ |
IL-1β | 5′-ATGCCACCTTTTGACAGTGATG-3′ | 5′-GCAGCCCTTCATCTTTTGGG-3′ |
IL-6 | 5′-CCTTCCAGGATGAGGACATGA-3′ | 5′-TGAGTCACAGAGGATGGGCTC-3′ |
Arg1 | 5′-TCACCTGAGCTTTGATGTCG-3′ | 5′- CTGAAAGGAGCCCTGTCTTG-3′ |
MCP-1 | 5′-CCCCAAGAAGGAATGGGTCC-3′ | 5′-GTGCTGAAGACCTTAGGGCA-3′ |
Ly6C | 5′-ACTGTGCCTGCAACCTTGT-3′ | 5′-GCTGGGCAGGAAGTCTCAAT-3′ |
CCR2 | 5′-ATCCACGGCATACTATCAACATC-3′ | 5′-CAAGGCTCACCATCATCGTAG-3′ |
iNOS | 5′-CAAGCACCTTGGAAGAGGAG-3′ | 5′- AAGGCCAAACACAGCATACC-3′ |
GAPDH | 5′-GCGAGATCCCGCTAACATCA-3′ | 5′-CTCGTGGTTCACACCCATCA-3′ |