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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Potential link between the RagA-mTOR-p70S6K axis and depressive-behaviors during bacterial liposaccharide challenge

Fig. 4

Immunofluorescence staining of LPS-induced mTORC1 translocation to the lysosomal membrane. a Neural stem cells were treated with 200 ng/ml of LPS, 20 nM Rapamycin (R), and 1 μM Temsirolimus (T) for 24 h. Following the treatment, the cells were stained with specific antibodies against mTOR and LAMP2, while the cell nuclei were indicated by DAPI staining. The stained cells were imaged on a Zeiss fluorescence microscope (Carl Zeiss, Jena, Germany). b Quantitative analysis of mTOR and LAMP2 levels. Co-localization of mTOR and LAMP2 signals in the neuronal stem cells was quantified by a densitometric method and expressed in mean ± SD (n = 3). The difference between the experimental groups was analyzed by one-way ANOVA, followed by post hoc Tukey’s test. *p < 0.05; ***p < 0.001 (LPS vs control/LPS + R/LPS + T)

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