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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Potential link between the RagA-mTOR-p70S6K axis and depressive-behaviors during bacterial liposaccharide challenge

Fig. 6

RagA silencing blocked LPS-induced translocation of mTORC1 towards lysosomal membrane. a After siRNA transfection, PC12 cells were treated with 1 μg/ml of LPS for 24 h, and stained with specific antibodies against mTOR and LAMP2, while the cell nuclei were indicated by DAPI staining. The stained cells were imaged on a Zeiss LSM 700 confocal fluorescence microscope (Carl Zeiss, Jena, Germany). b Quantitative analysis of mTOR and LAMP2 levels. Co-localization of mTOR and LAMP2 signals in the PC12 cells was quantified by a densitometric method and expressed in mean ± SD (n = 3). The difference between the experimental groups was analyzed by one-way ANOVA, followed by post hoc Tukey’s test. *p < 0.05; **p < 0.01; ***p < 0.001 (LPS vs control/ LPS + RagA siRNA or LPS + RagA siRNA vs LPS + negative siRNA)

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