Fig. 2

Quercetin inhibited necropotosis of OLs after SCI. a, c, e Double-immunostaining of CC1 with RIP3, MLKL, or pMLKL in saline or quercetin-treated rats at 10 dpi. Representative images are from sections 100 μm to the lesion epicenter. Arrows indicate double-positive cells. Scale bar = 30 μm. b, d, f Quantification of CC1/RIP3-, CC1/MLKL-, and CC1/pMLKL-positive cells. Notice the decrease of double-positive cells in quercetin-treated rats. *p < 0.05 compared to SCI + saline control. g, h Immunostaining and quantification of CC1-positive cells in sham, saline, or quercetin-treated rats at 10 dpi. Scale bar = 100 μm. Notice the increase of CC1-positive cells in quercetin-treated rats, compared to saline-treated rats. All data are expressed as mean ± SEM. Differences among groups were determined with unpaired two-tailed t test (b, d, f) or one-way ANOVA followed by Tukey post-hoc test (h). N = 6 in each group, *p < 0.05, ** p<0.01